Takara FFPE DNA QC All in One Kit
The Takara FFPE DNA QC All-in-One Kit combines qPCR-based quantification and fragmentation assessment in a single workflow. Using matched 92 bp (short) and 268 bp (long) assays targeting a human housekeeping gene, the kit calculates a long/short ratio that reflects DNA integrity and returns an absolute copy number of amplifiable molecules from as little as a few nanograms of input. Results provide clear guidance on DNA input and amplification cycles for downstream library prep kits such as the ThruPLEX and ThruPLEX FLEX kits.
Overview
- Two‑in‑one QC—performs simultaneous quantification and fragmentation evaluation by real‑time PCR; no electrophoresis required
- Minimal sample requirement—requires only a few ng of FFPE DNA vs. >100 ng typically needed for electrophoretic methods
- Actionable long/short ratio—uses 268 bp/92 bp assays to deliver a numeric integrity score that correlates tightly with DNA integrity number (DIN; R = 0.99)
- Proven library success—enables the use of the kit’s copy number and integrity data to adjust input or cycle number and obtain uniform, high-yield libraries with ThruPLEX Tag-Seq FLEX
- All-inclusive and instrument-flexible formulation—includes premix, standards, and ROX; validated on common real-time PCR platforms (Thermal Cycler Dice, CFX96, LightCycler 96/480, QuantStudio 5, etc.)
More Information
Applications
- Prelibrary QC of FFPE-derived DNA for Illumina and other NGS workflows
- Optimization of input amount and PCR cycles for ThruPLEX, ThruPLEX Tag‑Seq FLEX, and related library prep kits
- Quality assessment of archival or highly fragmented clinical research samples
- Biobank sample triage to maximize sequencing success and control costs
- Method development or validation studies requiring quantitative measurement of FFPE DNA integrity
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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